Research Journal of Pharmacology and Pharmacodynamics

Nitin Mahurkar ¹, S. M. Sayeed Ul hasan ¹, A. Arati Malpani ¹, Chetan Manjunath ¹

Affiliations
1 HKES College of Pharmacy, Sedam Road, Gulbarga–585 105 Karnataka, IN

Abstract

Aim: To evaluate the gastroprotective effect of the antioxidants (vitamin C and melatonin), rabeprazole and their combination in ethanol induced peptic ulcers.
Methodology: The study was divided into 2 dosage groups-single and multiple dose study groups. Each of the dosage group consists of wistar albino rats of either sex (180-220g) divided into 4 groups of 6 animals each.
In single dose study, The animals were fasted for 48h. At the end of 48h, the animals were administered with the drugs. 1h after drug administration, absolute ethanol (1ml/200g p.o.) was administered to all groups. 2h after ethanol administration, the animals were euthantised and stomachs were excised out. In multiple dose study, the animals were administered with drugs for 7 consecutive days. On 7th day following drug administration, the animals were fasted for 48h. At the end of 48h, the aimals were administered with absolute ethanol (1ml/200g p.o.) 2h after ethanol administration, the animals were euthantised, stomachs were excised out. The stomachs excised out from both the study groups were evaluated for ulcer index and percentage protection. However, for multiple dose study group histopathological study was also performed to evaluate cytoprotection.
Result: The results were interpreted statistically using one way ANOVA. The combination group (rabeprazole + vitamin C and rabeprazole + Melatonin) showed significant antiulcer activity in both single and multiple dose study groups, on comparison with rabeprazole, vitamin C and melatonin individually.
Conclusion: The effect of combination groups was found to be synergistic in nature and more effective in treating peptic ulcer disease.

Keywords

Antiulcer activity, Rabeprazole, Vitamin C, Melatonin, Antioxidants, Ethanol induced ulcers

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M. Nitin ¹, U. Mohan Reddy ², S. M. Sayeed Ul Hasan ²

Affiliations
1 Department of Pharmacology, H.K.E.S's, Matoshree Taradevi Rampure Institute of Pharmaceutical Sciences, Sedam Road, Gulbarga- 585 105, Karnataka, IN
2 H.K.E.S's, Matoshree Taradevi Rampure Institute of Pharmaceutical Sciences, Sedam Road, Gulbarga- 585 105, Karnataka., IN

Abstract

Gastric ulcer is one of the most prevalent gastrointestinal disorders, which affects approximately 5-10% of people during their life. The present study was aimed to find out the pharmacodynamic influence of Vitamin C and Esomeprazole and their combination on Gastro intestinal parameters in pylorus ligation and aspirin induced ulcers in rats. In pylorus ligation induced ulcer model, various parameters were studied viz. gastric volume, pH, total acidity, free acidity, and ulcer index. Ulcer index and percentage inhibition of ulceration was determined for aspirin induced ulcer model. The antiulcer effect of the combination of vitamin C 4.5 mg/200 g and esomeprazole 0.54 mg/200 g b.w orally was compared with the reference standard esomeprazole 0.54 mg/200 g b.w orally. The ulcer index was calculated and other biochemical parameters of gastric juice were estimated. The ulcer index of combination showed significant (P < 0.05) reduction while other biochemical parameters like volume, pH, free acidity and total acidity of gastric juice showed highly significant (P < 0.001) reduction when compared to control and standard esomeprazole. The percentage protection of combination was 85% as compared to standard esomeprazole 80% in pylorus ligation model. In the aspirin induced model the percentage protection of combination was 90% as compared to standard esomeprazole 85%. Thus the combination group was found to be synergistic in nature when compared to esomeprazole alone in both the models.

Keywords

Antiulcer Activity, Vitamin C, Esomeprazole, Pylorus Ligation, NSAIDs, Ulcer Index.

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M. A. Halkai ¹, Nitin Mahurkar ², M. D. Akhil Ahmed ³, A. K. Beknal ¹, S. M. Sayeed Ul Hasan ², M. Swathi ¹

Affiliations
1 Department of Pharmacognosy, H.K.E.'s MTR IPS, Gulbarga - 585 105, IN
2 Department of Pharmacology, H.K.E.'s MTR IPS, Gulbarga - 585 105, IN
3 Department of Pharmacognosy, H.K.E.'s MTR IPS, Sedam Road, Gulbarga - 585 105, IN

Abstract

Objective

To evaluate the gastroprotective effect of aqueous extract of leaves of Commicarpus chinensis in pylorus ligated albino rats.

Materials and Methods

The plants were collected from the campus Gulbarga University, Gulbarga, Karnataka and authenticated by department of Botany, Gulbarga University, Gulbarga. The leaves were shade dried and subjected to extraction by percolation to obtain aqueous extract. The aqueous extract was evaluated for antiulcer activity by pylorus ligation model in albino rats. Albino rats of either sex weighing between 180-220 g were procured from the Central animal house, MR medical college, Gulbarga, Karnataka. The animals were divided into three groups of six animals in each. The animals were fasted for 48h and subjected to pylorus ligation technique for evaluating the antiulcer effect of C. chinensis. Prior approval of the IAEC was obtained for conduction of experiment.

Results

The aqueous leaf extract showed significant antiulcer activity on comparison with that of standard rabeprazole. The aqueous extract reduced the acidity of gastric juice and increased the pH of the gastric secretions, exhibiting statistically significant ulcer protection activity.

Conclusion

The antiulcer effect of C. chinensis may be due to antioxidant property contributed by the presence of tannins, flavonoids, saponins. Thus, C. chinensis possesses effective antiulcer property.

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Keywords

Commicarpus Chinesis, Pylorus Ligation, Rabeprazole, Antiulcer Activity.

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